directional cloning

directional cloning
The technique by which a vector and a DNA insert are both digested with two different restriction endonucleases to create non-complementary sticky ends at either end of both molecules, so favouring the insert to be ligated into the vector in a specific orientation, while also preventing the vector from re-circularizing.

Glossary of Biotechnology for Food and Agriculture . 2015.

Игры ⚽ Поможем сделать НИР

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