restriction endonuclease

restriction endonuclease
A class of enzymes that cut DNA after recognizing a specific sequence. The three types of restriction endonuclease are: I. Where the cut occurs within a random sequence at sites >1kbp from the recognition sequence, and has both restriction and methylation activities. II: Cuts within, or near a short, usually palindromic recognition sequence. A separate enzyme methylates the same recognition sequence. III: Cuts 24-26bp downstream from a short, asymmetrical recognition sequence, requires ATP and has both restriction and methylation activities. Type II enzymes are the class used for most molecular biology applications.

Glossary of Biotechnology for Food and Agriculture . 2015.

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  • restriction endonuclease — noun any of the enzymes that cut nucleic acid at specific restriction sites and produce restriction fragments; obtained from bacteria (where they cripple viral invaders); used in recombinant DNA technology • Syn: ↑restriction nuclease,… …   Useful english dictionary

  • Restriction endonuclease — An enzyme from bacteria that can recognize specific base sequences in DNA and cut (restrict) the DNA at that site (the restriction site). Also called a restriction enzyme. * * * restriction endonuclease n RESTRICTION ENZYME * * * see restriction… …   Medical dictionary

  • restriction endonuclease — restriktazė statusas T sritis chemija apibrėžtis Fermentas, specifiškai skaidantis DNR. atitikmenys: angl. restrictase; restriction endonuclease rus. рестриктаза …   Chemijos terminų aiškinamasis žodynas

  • restriction endonuclease — restriction enzyme …   The new mediacal dictionary

  • restriction endonuclease — noun see restriction enzyme …   New Collegiate Dictionary

  • restriction endonuclease —    Enzymes (molecular scissors) that cleave double stranded DNA at specific palindromic base recognition sequences. The sequences are usually different for each enzyme. Restriction enzymes are named according to the bacterial species of origin …   Forensic science glossary

  • DpnII restriction endonuclease family — DpnII Identifiers Symbol DpnII Pfam PF04556 Pfam clan CL0236 …   Wikipedia

  • RecA-assisted restriction endonuclease cleavage — RecA assisted restriction endonuclease cleavage. См. метод RARE расщепления. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • restriction fragment length polymorphism — n variation in the length of a restriction fragment produced by a specific restriction enzyme acting on DNA from different individuals that usu. results from a genetic mutation (as an insertion or deletion) and that may be used as a genetic… …   Medical dictionary

  • restriction fragment — n a segment of DNA produced by the action of a restriction enzyme on a molecule of DNA * * * a DNA fragment produced by a restriction endonuclease …   Medical dictionary

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