nick translation

nick translation
A procedure for labelling DNA by treating a fragment with DNAse to produce single-stranded nicks followed by excision of a nucleotide and repair of the gaps with radiolabelled nucleotide.

Glossary of Biotechnology for Food and Agriculture . 2015.

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  • Nick translation — Nick translation(or Head Translation) was developed in 1977 by Rigby and Paul Berg. It is a tagging technique in molecular biology in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues …   Wikipedia

  • nick translation — noun A method of radioactively labelling a DNA molecule • • • Main Entry: ↑nick …   Useful english dictionary

  • nick-translation — nick translation. См. смещение разрыва. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Nick translation — Als Nick Translation wird in der Molekularbiologie eine DNA Markierungstechnik bezeichnet. Dabei nutzt man die Reparaturfunktion der DNA Polymerase I aus Escherichia coli aus, um markierte Nukleotide in Einzelstrangbrüche, sogenannte nicks… …   Deutsch Wikipedia

  • nick translation — A technique used to radioactively label DNA. E.coli DNA polymerase I will add a nucleotide, copying the complementary strand, to the free 3 OH group at a nick, at the same time its exonuclease activity removes the 5 terminus. The enzyme then adds …   Dictionary of molecular biology

  • nick translation — a process by which radiolabeled nucleotides are incorporated into duplex DNA at single strand nicks or cleavage points created enzymatically along its two strands …   Medical dictionary

  • Nick Pollotta — is an American author. The majority of his work has been published by Gold Eagle Books under house names James Axler and Don Pendelton . He lives in Chicago with his wife, Melissa. Contents 1 Bibliography 1.1 1989 1.2 1990 …   Wikipedia

  • Nick Palmer — In office 1 May 1997 – 12 April 2010 Preceded by Jim Lester Succeeded by …   Wikipedia

  • Nick Darke — born Nicholas Temperley Watson Darke (29 August 1948 10 June 2005) was best known as playwright but was also a writer, poet, lobster fisherman, environmentalist, beachcomber, politician, broadcaster, film maker and chairman of St Eval Parish… …   Wikipedia

  • Nick Mamatas — Born February 20, 1972 (1972 02 20) (age 39) Long Island, New York Occupation Novelist, Short Story Author, Essayist, Editor Nationality United States Genres horror …   Wikipedia

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