Southern hybridization

Southern hybridization
A procedure in which a cloned, labelled segment of DNA is hybridized to DNA restriction fragments on a Southern blot.

Glossary of Biotechnology for Food and Agriculture . 2015.

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  • reverse Southern hybridization — a variation on a Southern blot (q.v.) for detection of specific DNA sequences, in which DNA sequences in solution are first hybridized to a labeled probe and then separated electrophoretically …   Medical dictionary

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  • Southern blot (blot analysis blot hybridization) — South·ern blot (blot analysis, blot hybridization) (suthґərn) [E.M. Southern, British biologist, born 1938] see under blot …   Medical dictionary

  • Southern Blot — Autoradiogramm eines Southern Blots Beim Southern Blot handelt es sich um eine 1975 von Edwin Southern entwickelte molekularbiologische Untersuchungsmethode für die DNA.[1] Sie ermöglicht den Nachweis einer Gensequenz in einem komplexen DNA… …   Deutsch Wikipedia

  • Southern blot — A test commonly used in molecular biology and genetics, the purpose of the test being to check for a match between DNA molecules. In the Southern blot technique, DNA fragments are separated by a method called agarose gel electrophoresis,… …   Medical dictionary

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  • Southern blotting technique — The procedure used to isolate and identify DNA fragments from a complex mixture. The isolated, denatured fragments are transferred from an agarose electrophoretic gel to a nitrocellulose filter and identified by hybridization with probes …   Dictionary of microbiology

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