polyacrylamide gel electrophoresis

polyacrylamide gel electrophoresis
Abbreviation: PAGE
Ubiquitous method for separating nucleic acids and proteins on the basis of their molecular size. The method relies on the migration through an inert matrix (polyacrylamide gel) of electrically charged molecules as a result of the imposition of an electric field.

Glossary of Biotechnology for Food and Agriculture . 2015.

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  • polyacrylamide gel electrophoresis — polyacrylamide gel electrophoresis. См. электрофорез в полиакриламидном геле. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • polyacrylamide gel electrophoresis — (PAGE) gel electrophoresis using a polymerized polyacrylamide matrix to separate molecules on the basis of size, charge, or both; usually used to separate proteins or sequence nucleic acids. Gels are usually discontinuous (see disc e.) but may be …   Medical dictionary

  • polyacrylamide gel electrophoresis — (= PAGE) Analytical and separative technique in which molecules, particularly proteins, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the… …   Dictionary of molecular biology

  • SDS–polyacrylamide gel electrophoresis — (SDS PAGE) a type of polyacrylamide gel electrophoresis in which the anionic detergent sodium dodecyl sulfate (SDS) is used to denature the sample proteins into linear monomers, rendering their charge proportional to their length so that… …   Medical dictionary

  • polyacrylamide gel electrophoresis — (PAGE)    Used to identify size differences in VNTRs …   Forensic science glossary

  • Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

  • Gel electrophoresis — electrophoresis electrophoresis n. 1. (Chem.) the motion of charged molecules or particles in a liquid medium under the influence of an electric field; particles with a positive charge move toward the cathode and negative to the anode. [WordNet… …   The Collaborative International Dictionary of English

  • Polyacrylamide gel — A Polyacrylamide Gel is a separation matrix used in electrophoresis of biomolecules, such as proteins or DNA fragments. Traditional DNA sequencing techniques such as Maxam Gilbert or Sanger methods used polyacrylamide gels to separate DNA… …   Wikipedia

  • polyacrylamide gel — n hydrated polyacrylamide that is used esp. to provide a medium for the suspension of a substance to be subjected to gel electrophoresis …   Medical dictionary

  • gel electrophoresis — Electrophoresis using a gel supporting phase. Usually applied to systems where the gel is based on polyacrylamide. See electrophoresis …   Dictionary of molecular biology

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