iso-electric focusing gel

iso-electric focusing gel
Abbreviation: IEF gel
A variant of gel electrophoresis, in which macromolecules (usually proteins) are separated on the basis of differing iso-electric point, rather than on the basis of size.

Glossary of Biotechnology for Food and Agriculture . 2015.

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  • Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

  • electrophoresis — A ubiquitous molecular biology technique, with many variants, used to resolve complex mixtures of macromolecules into their components. Its principle is to subject samples to an electric field applied across a porous matrix. Molecules will… …   Glossary of Biotechnology

  • isoelectric — Of equal electrical potential. Cf.:i. point. SYN: isopotential. i. focusing electrophoresis of small molecules or macromolecules in a pH gradient. * * * iso·elec·tric .ī sō i lek trik adj 1) having or representing the same or connecting points… …   Medical dictionary

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