ligase chain reaction

ligase chain reaction
Abbreviation: LCR
A technique for the detection and amplification of target DNA sequences. Two oligonucleotides are synthesized which between them are complementary to the entire target sequence, one to the 5'-side and one to the 3'-side. If the target sequence is present in the DNA sample under examination, the oligonucleotides will bind to it with their ends abutting in the centre, and a heat-stable ligase will join them into a complete polynucleotide. No ligation occurs if the target sequence is absent or if the match between synthetic oligonucleotides and target sequence is imperfect in the region where they abut. At a high temperature, the new polynucleotide dissociates from the original DNA template, and upon cooling, it and the original DNA serve as templates for a second cycle of hybridization, ligation and thermal dissociation. At each cycle there is a doubling of the number of new complete polynucleotides.

Glossary of Biotechnology for Food and Agriculture . 2015.

Игры ⚽ Поможем написать курсовую

Look at other dictionaries:

  • Ligase chain reaction — The Ligase chain reaction (LCR) is a method of DNA amplification. While the better known PCR carries out the amplificiation by polymerizing nucleotides, this method instead amplifies the nucleic acid used as the probe. For each of the two DNA… …   Wikipedia

  • ligase chain reaction — a type of DNA amplification technique that uses DNA ligase to link adjacent pairs of synthetic oligonucleotides that have been hybridized to complementary regions on each strand of the double stranded target DNA, with the product used as a… …   Medical dictionary

  • Polymerase Chain Reaction — Die Polymerase Kettenreaktion (englisch Polymerase Chain Reaction, PCR) ist eine Methode, um die Erbsubstanz DNA in vitro zu vervielfältigen. Dazu wird ein Enzym verwendet, die DNA Polymerase. Der Begriff Kettenreaktion beschreibt in diesem… …   Deutsch Wikipedia

  • Ligase-Kettenreaktion — Die Ligase Kettenreaktion (englisch Ligase Chain Reaction, LCR) ist seit 1989 ein Nachweisverfahren für geringste Mengen von Erbmaterial (DNA). Sie funktioniert ähnlich wie die Polymerase Kettenreaktion, nur unter Verwendung eines anderen Enzyms… …   Deutsch Wikipedia

  • reaction — 1. The response of a muscle or other living tissue or organism to a stimulus. 2. The color change effected in litmus and certain other organic pigments by contact with substances such as acids or alkalies; also the property that such substances… …   Medical dictionary

  • Butyrate-CoA ligase — In enzymology, a butyrate CoA ligase (EC number|6.2.1.2) is an enzyme that catalyzes the chemical reaction:ATP + an acid + CoA ightleftharpoons AMP + diphosphate + an acyl CoAThe 3 substrates of this enzyme are ATP, acid, and CoA, whereas its 3… …   Wikipedia

  • Long-chain-fatty-acid-(acyl-carrier-protein) ligase — In enzymology, a long chain fatty acid [acyl carrier protein] ligase (EC number|6.2.1.20) is an enzyme that catalyzes the chemical reaction:ATP + an acid + [acyl carrier protein] ightleftharpoons AMP + diphosphate + acyl [acyl carrier protein]… …   Wikipedia

  • Long-chain-fatty-acid-luciferin-component ligase — In enzymology, a long chain fatty acid luciferin component ligase (EC number|6.2.1.19) is an enzyme that catalyzes the chemical reaction:ATP + an acid + protein ightleftharpoons AMP + diphosphate + an acyl protein thioesterThe 3 substrates of… …   Wikipedia

  • DNA ligase — DNA li·gase (liґgās) an enzyme of the ligase class that catalyzes the energy driven linkage of a double stranded DNA chain with a free 3′ hydroxyl group to one with a 5′ phosphate group, forming a phosphodiester bond between them …   Medical dictionary

  • Gene synthesis — is the process of synthesizing an artificially designed gene into a physical DNA sequence.Gene synthesis was first demonstrated by Har Gobind Khorana in 1970 for a short artificial gene. Nowadays, commercial gene synthesis services are available… …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”